A multi-parameter cell analyzer equipped with 4 excitation lasers and 18 detectors.

 

The system has the flexibility to support the expanding needs of multicolor flow cytometry assays. The BD LSRFortessa has an established platform for multicolor analysis with automatic compensation calculations.

In addition to manual tube loading, there is an option to use a high throughput system loader for 96 and 384 well plates. BD FACSDIVA software is used to acquire data and the exported data files can be analyzed in the FlowJo software in two independent working stations. The machine is very easy to handle and usually users work independently following short training.

BD LSR Fortessa configuration

Excitation Lasers

Emission Filters (nm)

Examples of Available dyes

Violet 405nm

670/30

Qdot655

610/20

BV605

585/15

Qdot585

540/30

Qdot565

525/50

AmCyan

450/50

DAPI, V450, Vio 405/452, BV421, Hoechst

Blue
488nm

710/50

PerCP cy5.5

530/50

FITC, GFP, YFP, Alexa488

488/10

SSC

Yellow-Green
561nm

780/60

PE-Cy7

710/50

PE-Cy5.5

670/30

PE-Cy5

610/20

mCherry, PI, Texas Red

586/15

PE, DsRed, RFP, Cy3

Red
640nm

780/60

APC-Cy7

730/45

Alexa700

670/14

APC