20220608105746.710.jpg

  • The Pre-clinical Research Center was inaugurated in 2015.
  • It is designed to accommodate mice in IVC cages under SPF conditions.
  • The services provided include breeding, quarantine, reverse light cycle, behavior tests, maintenance, procedure rooms, and an imaging center
  • Five rooms are designated as a professional area for investigators to carry out examinations, observations, manipulations, and procedure

Team

  • Head of the vivarium and vet – Simon Tinman
  • Vivarium responsible and animal caretaker – Meir Huta
  • Animal Technician (procedures) – Rivka Sabag
  • Washing area – Itamar Giladi
  • Animal caretakers – Bezalel Mishne and Itamar Nativ

 

Vivarium

  • Sensemode monitoring on line (alarms through email and cellular messages)
  • Temp: 20-24 C
  • RH: 35- 65%
  • Light: 12/12 (7:00 – 19:00 light)
  • HEPA filtered air (building)
  • IVC – Green line Teniplast cages
  • Irradiated pellets (food) – Altromin, Germany
  • Water – 4 filters (until 0.2 micron) + acidifying pH 2.8 + Chlorinator
  • All the equipment disinfected and Autoclaved
  • Biometry entrance
  • Internal doors: private doors
  • Video
  • Health monitoring: daily cage checks
  • FELASA Annual + another 1-2 quarterly FELASA + any unexpected case
  • Equipment entrance room: Dual chamber
  • Hydrogen peroxide – disinfection
  • Air flow – Hood for animals
  • Storage room – equipment, bedding, and disposables
  • Low Temperature Storage – Food
  • Animal care takers – Special clothes + Single use
  • Students – single use clothes on top
  • Gowns, shoe cover, head cover, mask, gloves
  • Air washer

 

Washing area

  • “Dirty” and Clean side
  • Cage change – Maximum every 10 days
  • Dirty bedding safety disposal
  • Washing machine for ages
  • Washing machine for bottles
  • Clean Bedding safety filling machine
  • Double door Tuttenauer autoclave

20220608110531.351.jpg


 

Maintenance rooms

  • 3 Maintenance room
  • 1 breeding room for immuno-deficient mice
  • IVC – positive pressure
  • Tecniplast – green line
  • 5 adults mice/ cage
  • Every cage – tag
  • Special tags: Solitary mice in cage, special food, overcrowding, suspected sick , injured mice; breeding cage, pups, etc.

20220608110627.008.jpg

For more information, please contact: Dr. Shimon Tinman Veterinarian and Head of the Animal Facilities Tel: 972-3-5318748; tinman@biu.ac.il

 Quarantine room

  • “clean quarantine”
  • Different colored cages
  • Negative pressure (room and rack)
  • Parallel passage to washing area
  • Last time of the day
  • Special entry permit
  • Different entrance

20220608110715.043.jpg


 

Procedure room

  • Big Biosafety hood
  • Anesthesia machine – Isoflurane (Chemical hood)
  • Freezer and refrigerator
  • CO2 Euthanasia chamber
  • Pos- op room
  • Blood Analyzers (Biochemistry and analyzer) room
  • Computer and Printer
  • Centrifuge, clipper, microscopes, magnifying lens, etc

 

20220608110811.990.png


 

Behavior room

  • Prof Eitan Okun
  • 1 maintenance Reverse light (20:00 – 8:00 Light)
  • 2 behavior rooms

20220608110907.275.jpg


 

Metabolic room

20220608110941.811.jpg


 

Imaging Room

  • MRI
  • Fluorescence in vivo – Maestro
  • X Ray
  • NMR
  • Blood pressure unit