About the Unit
The Transgenic Unit at Bar-Ilan University specializes in generating transgenic mice and advanced genome editing using cutting-edge technologies like CRISPR. Our unit collaborates with researchers across various fields, offering a comprehensive range of services in genomic editing—from project planning to the production of genetically tailored offspring. Led by Dr. Shelly Ziv-Lherman, the unit is dedicated to supporting research endeavors with precision and expertise.
Services Provided:
- Sperm Cryopreservation:
- Preservation of mice lines by sperm freezing and for live animal reduction.
- Import of frozen sperm from international or other academic sources for the creation of clean new mouse strains.
- In Vitro Fertilization (IVF):
- Generation of mouse lines from cryopreserved strains via in vitro fertilization (IVF).
- Mice rederivation services:
- Mice rederivation through cesarean section and foster mother transfer, ensuring a healthy and uncontaminated lineage.
- Embryo Transfer:
- Embryo transfer into recipient females to produce a genetically engineered mice offspring.
- Genome Editing via CRISPR:
- Creating mutations through indels and generating genomic deletions.
- Insertion of desired sequences (up to 200 base pairs), such as tags or amino acid changes.
- Development of Founder mice with precise genetic modifications.
- Insertion of Large Genomic Fragments:
- Utilizing AAV technology to insert fragments up to 4.4Kb, expanding the possibilities of genetic engineering.
Consultation and Guidance: The unit offers personalized consultation on genome editing strategies, guide design, and HDR oligo planning under the guidance of Dr. Nitzan Gonen, an expert in genomic technologies.
Our goal is to provide tailored solutions to meet the specific needs of each research project.