Opera Phenix® Plus is a high throughput microplate confocal imager for High-Content Screening (HCS). It can acquire, analyze and manage fluorescence, brightfield and digital phase contrast images.

 

Configuration

  • Two sCMOS cameras:
  • 7 mega pixel (2160x2160), 16 bit resolution, 6.5 μm pixel size
  • Simultaneous acquisition of up to four channels with minimized crosstalk
  • Each camera supports fast recording mode with up to 100 fps

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  • Proprietary Synchrony Optics combine a microlens-enhanced Nipkow spinning disk with dual view confocal optics to separate fluorescence excitation and emission during simultaneous aquisition
  • Excitation Configurations with 5 lasers - 375 nm 50mW, 425 nm 100mW, 488 nm 150mW, 561 nm 100mW, 640 nm 140mW.
  • Imaging Modality Selectable widefield and confocal imaging modes depending on the assay requirements
  • Microlens-enhanced dual spinning disk confocal unit with optimized pinhole-to-pinhole distance to further reduce out-of-focus noise in thick samples such as spheroids
  • Transmission brightfield and digital phase contrast
  • Emission Filter 435-515 nm, Typically used for CFP/YFP FRET studies
  • Air Objective:
    • 5x Air objectiveNA 0.16, WD 12.1 mm
    • 10x Air objective NA 0.3, WD 5.2 mm
    • 20x Air, NA 0.4, WD 8.28 mm
  • Water immersion objectives – provides an improved light collection efficiency:
    • 20x Water Immersion Objective NA 1.0, WD 1.7 mm
    • 40x Water Immersion Objective NA 1.1, WD 0.62 mm
    • 63x Water Immersion Objective NA 1.15, WD 0.6 mm
  • Sample chamber with temperature, CO2 and humidity control to enable live cell experiments.
  • Compatible with various well plates – 96, 384 etc.
  • Slide Holder (up to 4 slides) x 4

LIVE CELL IMAGING PACKAGE

  • Plate handler FLEX 750 collaborative robot including control PC, Barcode reader, plateworks scheduling software
  • 14 position plate shelf
  • Liconic STX44 IC SA Incubator (32-50O C, >95% RH, 0-10% CO2) with 2x22 position plate cassettes for 96/384w SW plates.

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Harmony® High-Content Imaging and Analysis Software

  • Advanced analysis features such as texture and STAR
    morphology analysis provide detailed descriptions of cellular phenotypes, enabling more robust and reproducible
    classification
  • 3D visualization and analysis features
  • Ready-made solutions and building blocks for image analysis
  • Includes 2x PhenoLOGIC™ - Machine Learning algorithm
  • Includes 2x PreciScan™ - Intelligent Acquisition plug-in whichdetermines the xyz-position of the object and use it in a re-scan.
  • Includes Cell Painting

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