Introduction:

The Leica 3D STED system achieves resolution below the diffraction limit in both the lateral, and axial directions. Our Leica SP8 offers one of the only tunable white light lasers in Israel. There are two STED continuous wave lasers at 592 nm and 660 nm which allow the use of fluorophores up to 595nm in super-resolution mode.

Overview:

The SP8 has been designed for confocal microscopy with optimal photon efficiency and high speed. All optical components are matched towards preserving fluorescence photons for image contrast and to improve cell viability in live cell imaging.

Specifications:

  • Inverted Leica DMi8 with anti-vibration table
  • Z-Galvanometer
  • Continuous wave laser: UV- Diode 50mW 405nm
  • Pulsed laser: White Light Laser (WLL).   power 1.5mW:470 - 670nm
  • Depletion lasers: two continuous (CW) wave lasers at 592 nm and 660 nm
  • Spectral Detection Range of 400nm - 720nm
  • Detectors: two HyD (hybrid detectors), two PMTs, and a TLD (transmitted light detector)
  • Excitation Modulation: AOTF: up to 8 channels
  • Resonant Scanner 8kHz (Maximal Frame Resolution: 1024x1024 pixel; Scan-Zoom: 1.3 - 48x. Up to 7 FPS at 512x512, up to 84 FPS at 512x16)
  • Accelerated Z-stacking with the SuperZ galvanometer substage

Objectives:

HC PL FLUOTAR 10x/0.30
Plan Fluotar objective, for use with and without coverglass, no immersion.
Free working distance: 11.0 mm.

HC PL APO 20x/0.75 (multi immersion)
Plan apochromatic multi-immersion objective.
Free working distance: 0.67 mm (water immersion and 0.17 mm coverglass).
Correction collar for use with and without coverglass and use with water, glycerine and oil immersion.

HC PL APO 40x/1.30 Oil CS2
Plan apochromatic oil immersion objective.
Free working distance: 0.24 mm.

HC PL APO 63x/1.40 OIL CS2
Plan apochromat oil immersion objective.
Free working distance: 0.14 mm.

HC PL APO 100x/1.40 Oil STED only
Plan apochromat oil immersion objective especially designed for use with STED and STED 3X. Axial color shift of objective <100 nm

http://www.leica-microsystems.com (Funding by ISF grant)

Leica Stellaris 5 live imaging (2021) Leica SP8 Confocal live imaging (2016)